Journal: Poultry Science
Article Title: Music intervention mitigates LPS-induced gut barrier disruption and immune stress in broilers via TLR4/NF-κB regulation
doi: 10.1016/j.psj.2025.105189
Figure Lengend Snippet: Music has potential anti-inflammatory effects and can effectively alleviate inflammation in the jejunum and ileum by inhibiting the LPS-triggered TLR4/NF-κB signaling pathway. (A) The figure shows the relative mRNA expression levels of the TLR4/NF-κB inflammatory signaling pathway and related inflammatory factors in the jejunum and ileum of broilers. (B) The figure presents the immunoblot images and corresponding statistical results of TLR4, IKB-α, NF-κB p65, and TNF-α proteins in the jejunum and ileum of broilers. (C) The figure shows the measurement results of IL-1β, IL-6, and IL-10 levels in the jejunum and ileum of broilers across different groups. Differences marked with lowercase letters denote a significant difference ( P < 0.05), while the same letter indicates no significant difference ( P > 0.05).
Article Snippet: The membrane was then blocked in 5 % skim milk at 37°C for 2 h. After blocking, the membrane was incubated with diluted primary antibodies against TLR4, IKB-α, NF-κB, and TNF-α (1:1000, Abmart, China) at 4°C for 12 h. The specific antibodies used are listed in STable 6 .
Techniques: Expressing, Western Blot